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Envelope incorporation into virions. Virions were pelleted, lysed, separated by SDS-PAGE, and visualized by Western blotting. (A) Western blot of viral pellets with anti-gp120 MAb and anti-p27 (capsid) MAb. (B) Relative Env incorporation into virions. The ratios of gp120 to p27 were calculated by using phosphorimaging analysis. Data are presented relative to the ratios found for SIV239 virions (Rn/R239).
