FIG. 3.
SARS-CoV induces expression of chemokines in A549 cells. (A) A549 cells were infected with SARS-CoV as described for Fig. 2. At different days after infection, total RNA was extracted and subjected to RPA and phosphorimaging. The intensities of the CCL2/MCP-1 and CXCL8/IL-8 mRNA phosphorimages were normalized against that of the L32 housekeeping gene. The data presented are representative of two independent experiments. (B) The supernatants from uninfected (left panel), SARS-CoV-infected (middle panel), or CoV-299E-infected (right panel) A549 cells were collected at day 1 after infection. The cytokines and chemokines secreted into the supernatants were determined on the protein chip. The positions of cytokines and chemokines on the array are shown. The cytokines/chemokines that were detected in the supernatant of SARS-CoV-infected cells are boxed. Those that were in the supernatant of CoV-229E-infected cells are circled.