Table 3.
PQE-1 proteins containing the Q/P-rich and charged domain suppress polyQ toxicity in pqe-1 animals
| Genotype |
Lines |
% affected at 3 days | %
affected, total |
|
|---|---|---|---|---|
| Degeneration | Death | |||
| pqe-1(rt13); Htn-Q150 | − | 3 | 97 | 100 |
| pqe-1(rt13); Htn-Q150; F52C9 | 7 | 11+/−5 | 30+/−5 | 56+/−14 |
| pqe-1(rt13); Htn-Q150; GFP | 4 | 7+/−3 | 93+/−4 | 99+/−1 |
| pqe-1(rt13); Htn-Q150; PQE-1A:GFP | 6 | 7+/−2 | 19+/−4 | 26+/−4 |
| pqe-1(rt13); Htn-Q150; PQE-1B:GFP | 4 | 4+/−2 | 96+/−9 | 100 |
| pqe-1(rt13); Htn-Q150; PQE-1C:GFP | 5 | 9+/−3 | 23+/−8 | 33+/−8 |
The genotype and transgene are listed in column 1; the number of transgenic lines scored is listed in column 2. Htn-Q150 was expressed from rtIs11, an array expressing Htn-Q150 and OSM-10:GFP by using the osm-10 promoter that has been integrated into chromosome V. The percentage of ASH neurons degenerating (dye-filling defective) but surviving (expressing osm-10:GFP) is reported in subcolumn 3. The percentage of dead ASH neurons (dye-filling defective and lacking GFP expression) is reported in subcolumn 4. The percentage of affected neurons (sum of subcolumns 3 and 4) is indicated. The cosmid F52C9 contains pqe-1(+). More than 150 ASH neurons were scored for each data point. The SEM is listed.