Fig. 6.
Susceptibility to serum deprivation-induced apoptosis in AMCs from ARDS-R vs. ARDS-NR. AMCs isolated from all 4 patients with ARDS-NR and 4 patients with ARDS-R were plated at an equal density in a 96-well plate. Cells were cultured in DMEM with 10% FBS for 4 days, and the medium was changed to DMEM with 0.1% FBS. Cells were cultured under serum-deprivation conditions for 6 days, and apoptosis was assessed with an ELISA for single-stranded (ss) DNA as described in materials and methods. P = 0.028 by nonparametric Mann-Whitney test; n = 4 for each AMC cell line. The experiment was repeated 3 times with similar results.