Fig 3.
GABAergic, cholinergic, and aminergic neurons express VGLUT3. (A–C) Scattered interneurons in stratum radiatum (Rad) of the hippocampus double stain for both VGLUT3 (A) and GAD (B and C). A subset of GAD-immunoreactive processes within the pyramidal cell layer (Py) also express VGLUT3. However, not all GAD-positive cells express VGLUT3 (arrow). (D–F) In the retina, a subset of cells in the inner nuclear layer (INL) and a discrete layer of synapses in the inner plexiform layer (IPL) label for VGLUT3 (D). Double staining for GAD (E) shows that the VGLUT3-positive cell bodies do not express GAD (F). (G–I) In the striatum, VGLUT3-immunoreactive perikarya and dendrites (G) also express choline acetyltransferase (H and I). (J–L) Processes of a raphe neuron grown in autaptic microculture label for VGLUT3 (J) as well as serotonin (K and L). Although most processes show colocalization, a subset contain serotonin but not VGLUT3 (arrow) whereas others contain VGLUT3 but not serotonin (arrowheads) (L). [Bars = 50 μm (A–C and G–I), 12.5 μm (D–F), and 10 μm (J–L).]