Fig 1.
Detection of spermatogenic cells in testis sections and cultures. (A Left) Hematoxylin- and eosin-stained section from testis of 22-day-old rat. (Center) Adjacent section labeled with anti-DAZL3 (green) plus anti-vimentin IgGs (red). (Right) Adjacent section labeled with preimmune (green) plus anti-vimentin IgGs (red). (Bar = 150 μm.) (B Upper Left) Testis cell culture from 22-day-old rat showing Dazl immunoreactivity (green) in germ cells on top of anti-vimentin-labeled somatic cells (red). (Upper Right) Preimmune (green) plus anti-vimentin IgGs (red). (Lower Left and Right) Bright-field microscopy of cultures at Upper Left and Right, respectively. (Bar = 40 μm.)