Fig 3.
Transcriptional activity of MRTFs. The complete ORFs (A) or TADs (B) of myocardin and MRTFs were fused to the GAL4 DNA-binding domain and tested for transcriptional activity by using a GAL4-dependent luciferase reporter (UAS-luc) in transfected COS cells. (C) Transactivation of luciferase reporters linked to SM22 or ANF promoters or four tandem copies of CArG-near from the SM22 promoter by myocardin and MRTFs, as indicated. WT refers to the wild-type promoter, and Mut refers to the promoters with mutations in the two CArG boxes. Values are presented as the fold activation of expression above the background level of expression of vector alone. All transfection assays were performed at least three times, and representative data are shown.