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SDS/PAGE analysis of drusen. Drusen isolated from a 93-year-old male, normal donor was dissolved in Laemmli sample buffer and ≈10 μg was fractionated by SDS/PAGE (4% stacking, 7.5% separating polyacrylamide gel). The gel was stained with Coomassie blue, bands were excised, and proteins were identified by LC MS/MS as described in Methods.
