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Subcellular localization of pp71 in HCMV infected cells. HFF cells were treated with 100 μg of cycloheximide/ml 30 min prior to infection (0.5 PFU/cell) and extending for 7 h after infection. Indirect immunofluorescence analyses were carried out with monoclonal antibodies directed against pp71 (a), pp65 (d) and IE1 (g). ND10 domains were visualized by staining the Sp100 protein (b, e, and h). DAPI staining of the respective cell nuclei is shown in panels c, f, and i.
