TABLE 1.
Addition of 6×His tag to AAV VP3 does not change AAV capsid viral tropisma
| Cell line | Mean no. of viral DNA particles/cell (103) after transfection with:
|
|
|---|---|---|
| rAAGFP-VP3.6×His | rAAVGFP | |
| 293 | 448 | 392 |
| HeLa | 392 | 312 |
| Skin fibroblast | 168 | 152 |
| Raji (B cell) | 3.2 | 0.8 |
| Jurkat (T cell) | 4.4 | 3.6 |
a
Five different human cell lines were transfected with equivalent numbers (500 TU/cell) of either rAAVGFP or rAAVGFP-VP3.6×His. The cells were harvested after 48 h, and the number of viral particles per cell was determined by DNA dot blot analysis as described elsewhere (33). Data are expressed as means of the results of three independent experiments.