Extended Data Fig. 6. Cryo-EM data processing of seeded mouse tau filaments.
A-D For AD and E-H for CBD seed-injected mice. A, E Representative cryo-EM micrographs. Scale bar, 50 nm. B, F Representative 2D class averages; the box sizes are 630 Å. C, G Fourier shell correlation curves (FSC) between two independently refined half-maps (black); between the atomic model fitted in half-map 1 and half-map1 (blue); between the atomic model fitted in half-map 1 and half-map 2 (yellow) and between the atomic model fitted in the sum of the two half-maps and the sum of the two half-maps (red). D, H Cross-sections through cryo-EM density maps of seeded mouse tau (top) and human brain-derived tau filaments (bottom). Similar additional densities at the periphery of the ordered cores are marked with arrows.