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IE and early (E) genes are per se sufficient to induce SA-β-Gal activity in infected cells. HELF were synchronized in G0 by serum starvation and treated with PFA immediately after HCMV infection (MOI, 5) or were not treated. (A) Histochemical staining for SA-β-Gal activity at 72 h p.i. (B) Immunohistochemical staining of IE (IE72 and IE86), E (UL44), and late (pp65) viral genes at 72 h p.i.. Magnification, ×20.
