FIG. 6.
Kinetics of virus replication and virus-specific CD8+-T-cell response in spleens of mice deficient in TNFR1, perforin and TNFR1, or perforin and IFN-γ following infection with a low dose of LCMV Docile. Mice were infected with 102 PFU of LCMV Docile, and virus titers in the spleens were measured at the time points indicated (A). The data shown are means ± standard errors of the means for 3 to 5 mice (in log10 PFU/gram of tissue). In addition, total numbers of GP133–41 or NP396–404 peptide-specific CD8+ T cells were measured by staining for H-2Db tetramer (filled circles) or intracellular IFN-γ (open circles) production following stimulation of cells with the appropriate peptide to determine the functional responsiveness of these cells (B and C). The data shown are means ± standard errors of the means for 3 to 5 mice (in log10 virus-specific T cells per spleen).