Figure 5.
Basal levels of phosphorylated HER3, EGFR (Y1068) c-MET and downstream molecules AKT, MAPK and STAT3 in parental BxPc3 cells and drug-resistant cell lines BxPc3AFR, BxPc3GEMR and BxPc3OSIR either grown in 10% FBS supplemented growth medium alone or with Afatinib, Crizotinib or their combination. Cells were grown to near confluence in 10% FBS at 37°C, and incubated overnight. Cells were then lysed, proteins were separated by SDS-PAGE, transferred to PVDF membranes and probed with the antibodies of interest. Representative of two independent experiments.