FIGURE 6.
Distribution of 11-nm particles in contracting intact ventricular myocytes. (A–C) Electron micrographs made using fast conventional fixation and silver enhancement. Representative micrographs show the distribution of particles before (A, 11-nm particles) and after (B, 11-nm, and C, 16-nm particles) 4-h pretreatment with 0.3 U/ml neuraminidase. M, mitochondrion; Z, Z-line. T-tubules are marked by arrows. (D) Graphs representing the density of 3- to 16-nm particles in spontaneously contracting intact ventricular cells before (gray) and after (dark gray) 4 h of pretreatment with 0.3 U/ml neuraminidase (n = 23–190). An asterisk indicates data that are statistically different from the corresponding control (P < 0.01). [Ca2+] = 5 mM.