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Dependence of SHG on myosin filaments. (A) SHG (purple) and fluorescence (green) from an isolated, unfixed mouse myofibril stained with AlexaFluor 488-phalloidin. (B) The same myofibril after myosin extraction. The SHG channel in panel B was collected with a fourfold increase in detector gain relative to panel A, but shows no sarcomeric signal. Scale bar = 5 μm.
