FIG. 2.
Association between RACK1 and MuV-V in MuV-infected cells. (A) Whole-cell lysates of FL cells (lane 1) and FLMT cells (lanes 2 and 3) were immunoprecipitated (IP) with anti-V protein antibody (lanes 1 and 3) or preimmune serum (Pre) (lane 2) and detected by Western blot (WB) analysis with anti-RACK1 antibody (upper panel) or anti-V protein antibody (lower panel). (B) FLMT cells were treated (+) or not treated (−) with IFN for 15 min, and then cell lysates were immunoprecipitated with anti-V protein antibody (V) (lanes 1 and 2) or anti-RACK1 antibody (R) (lanes 3 and 4) and subjected to immunoblotting with anti-RACK1 antibody (upper panel) or anti-V protein antibody (lower panel). (C) FL cells (lanes 1 and 2) and FLMT cells (lanes 3 and 4) were treated (+) or not treated (−) with IFN for 15 min, and then cell lysates were immunoprecipitated with anti-RACK1 antibody and analyzed by Western blotting with anti-STAT-1 antibody (upper panel) or anti-V protein antibody (lower panel). (D) FL cells (lanes 1 and 2) and FLMT cells (lanes 3 and 4) were treated (+) or not treated (−) with IFN for 24 h, and then the levels of production of RACK1 (upper panel) and MuV-V (lower panel) were investigated by immunoblotting.