Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| Male C57BL/6 mice (M. musculus), 8–12 weeks old | Originally obtained from Jackson Laboratory |
Strain # 000664 RRID:IMSR_JAX:000664 |
| LRH-1 VilCre and LRH-1 L2L2 mice (M. musculus), 8–12 weeks old | Previously described in Coste et al, 2007 | N/A |
| Caco-2 (H. sapiens) | ATCC | Cat# HTB-37 |
| HEK 293 T (H. sapiens) | ATCC | Cat# CRL-3216; RRID:CVCL_0063 |
| LS174T (H. sapiens) | ATCC | Cat# CL-188 |
| LS174T human Flag-tagged LRH-1, 4-HT-inducible | This study | N/A |
| LS174T human Flag-tagged LRH-1 F342W/I416W, 4-HT-inducible | This study | N/A |
| LS174T murine LRH-1, 4-HT-inducible | This study | N/A |
| LS174T EGFP, 4-HT-inducible | This study | N/A |
| Bifidobacterium animalis subsp. lactis 420 (B420TM) | Danisco Deutschland GmbH | Sample #767, Batch 1102133655 |
| Escherichia coli BL21 | Previous hosting lab | N/A |
| Escherichia coli Nissle 1917 (EcN) (Mutaflor) | Ardeypharm | N/A |
| Escherichia coli RosettaTM 2 (DE3) | Novagen | Cat# 71400 |
| Enterobacter cloacae | DSMZ | DSM No.: 30054 |
| Enterococcus faecalis | DSMZ | DSM No.: 20478 |
| Klebsiella pneumoniae | DSMZ | DSM No.: 681 |
| Pseudomonas aeruginosa PA01 | DSMZ | DSM No.: 22644 |
| Proteus mirabilis | DSMZ | DSM No.: 4479 |
| Symbioflor2 | Symbiopharm | N/A |
| Vibrio cholerae | DSMZ | DSM No.: 106276 |
| Recombinant DNA | ||
| Plasmids | Table 2 | |
| Antibodies | ||
| Mouse mAB anti-Flag M2 | Sigma-Aldrich | Cat# F1804 |
| Mouse mAB anti-GFP | Roche | Cat# 11814460001 |
| Mouse mAB anti-Tubulin | Sigma-Aldrich | Cat# T5168; RRID:AB_477579 |
| Rabbit pAB anti-NR5A2 | Sigma-Aldrich | Cat# HPA005455 |
| Oligonucleotides and other sequence-based reagents | ||
| Primers | Table 1 | |
| Chemicals, enzymes and other reagents | ||
| 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) | Sigma-Aldrich | Cat# M2128 |
| 4-hydroxytamoxifen | Sigma-Aldrich | Cat# H7904 |
| Adenosine 5-triphosphate (ATP) | Carl Roth | Cat# K029.2 |
| ammonium thiocyanate | Sigma-Aldrich | Cat# 1012130500 |
| AMPure XP | Beckman Coulter | Cat# A63881 |
| Cis-vaccenic acid | Sigma-Aldrich | Cat# V0384 |
| Cis-vaccenic acid | Cayman chemical | Cat# 20023 |
| Co-Enzyme A | PJK | Cat# 102211 |
| cOmplete TM, EDTA-free Protease Inhibitor Cocktail | Roche | Cat# 04693132001 |
| Compound 3d2 (3d2) | ChemBridge Corp | ID: 16728690 |
| Direct Red 80 | Sigma-Aldrich | Cat# 365548 |
| D-Luciferine Na salt (Luciferin) | PJK | Cat# 102132 |
| DNase I (RNase-free) | New England Biolabs | Cat# M0303 |
| Dulbecco’s Modified Eagle’s Medium | Sigma-Aldrich | Cat# D5796 |
| Fetal Bovine Serum | Sigma-Aldrich | Cat# F7524 |
| Gentamicin | Sigma-Aldrich | Cat # G1272 |
| Hygromycin B | InvivoGen | Cat# ant-hg-1 |
| Iscove’s Modified Dulbecco’s Medium | Sigma-Aldrich | Cat# I3390 |
| Isopropyl β-D-1-thiogalactopyranoside (IPTG) | Carl Roth | Cat# 2316.3 |
| JetPeiTM | Polyplus Transfection | Cat# 101000020 |
| L-glutamine | Sigma-Aldrich | Cat# G7513 |
| Luminol | Fluka | Cat# 09253 |
| Oil Red O | Sigma-Aldrich | Cat# O0625 |
| O-nitrophenyl-b-D-galactopyranoside (ONPG) | Carl Roth | Cat# CN.22 |
| P-coumaric acid | Sigma-Aldrich | Cat# C-9008 |
| PeqGOLD TriFastTM | VWR | Cat# 30–2030 |
| Puromycin. 2HCl | Enzo Life Sciences | Cat# BML-GR312 |
| SR1848 | Sigma-Aldrich | Cat# SML 1513 |
| TEV-protease | Sigma-Aldrich | Cat# T4455 |
| Tissue-Tek O.C.T Compound | Sakura | Cat# 4583 |
| Trans- vaccenic acid | Sigma-Aldrich | Cat# V1131 |
| TRIzolTM Reagent | Invitrogen | Cat# 15596026 |
| Software | ||
| Bioanalyzer Smear Analysis tool | Agilent Technologies | N/A |
| MO.Affinity Analysis software | NanoTemper Technologies | version 2.2.4 |
| NextSeq System Suite | Illumina Inc | v2.2.0 |
| Prism | GraphPad Software | V7.0 |
| QuantStudio Design & Analysis Software | Applied Biosystems | V1.5.2 |
| R package “EnhancedVolcano” | version 1.18.0 | |
| R package clusterProfiler | version 4.8.3 | |
| R package DESeq2 | version 1.40.2 | |
| R package pheatmap | version 1.0.12 | |
| ZEN Software | Carl Zeiss Microscopy | V2.5 (blue edition) |
| Other | ||
| 10 kDA Amicon Ultra tube | Millipore | Cat# UFC901024 |
| High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Cat# 4368814 |
| High-fat diet with 45% calories from fat | Safe lab | SAFE 245HF, D12451 |
| HiLoad 16/60 Superdex® 200 PG column | Cytiva | Cat# 28-9893-35 |
| HisTrap HP column | Cytiva | Cat# 29-0510-21 |
| Monolith NT Protein Labeling Kit GREEN-NHS | NanoTemper Technologies | Cat# MO-L002 |
| Monolith NT.115 capillaries | NanoTemper Technologies | Cat# MO-K022 |
| PD SpinTrap G-25 column | Cytiva | Cat# 28-9180-04 |
| Pierce™ BCA Assay Kit | Thermo Fisher | Cat# 23225 |
| PVDF membrane | Roche | Cat# 03010040001 |
| QuikChange II XL Site-Directed Mutagenesis Kit | Agilent Technologies | Cat# 200521 |
| Standard diet | Safe lab | SAFE A03 |
| SV Total RNA Isolation System | Promega | Cat# Z3100 |
| SYBR® Green | Applied Biosystems | Cat# 4385614 |
| Triglyceride Quantification Kit | Sigma-Aldrich | Cat# MAK266 |
| TruSeq® Stranded mRNA Sample Preparation Kit | Illumina Inc | Cat# 20020594 |
| Ultra Sensitive Mouse Insulin ELISA Kit | CrystalChem | Cat# 90080 |
| Venor®GeM Classic | Minerva Biolabs | Cat# 11-1050 |
| 3.5 KDa Slide-A-Lyzer MINI Dialysis Device | ThermoFisher Scientific | Cat# 88403 |
| Bioanalyzer 2100 | Agilent Technologies | N/A |
| BioAnalyzer 4200 tapeStation | Agilent Technologies | N/A |
| Glucometer | GlucoCheck | N/A |
| Hydrax C500 cryostat | Carl Zeiss | N/A |
| Image Quant LAS4000 | GE Healthcare | N/A |
| Infinite® 200 PRO plate reader | TECAN | N/A |
| Monolith NT.115 instrument | NanoTemper Technologies | N/A |
| NextSeq500 System | Illumina Inc | N/A |
| Reflotron Plus | Roche | N/A |
| QuantStudio 3 Real-Time-PCR-System | Applied Biosystems | N/A |
| Zeiss Observer Z.1 microscope | Carl Zeiss | N/A |