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. 2026 Sep 17;18(10):4059–4089. doi: 10.1038/s44321-026-00516-3

Reagents and tools table

Reagent/resource Reference or source Identifier or catalog number
Experimental models
Male C57BL/6 mice (M. musculus), 8–12 weeks old Originally obtained from Jackson Laboratory

Strain # 000664

RRID:IMSR_JAX:000664

LRH-1 VilCre and LRH-1 L2L2 mice (M. musculus), 8–12 weeks old Previously described in Coste et al, 2007 N/A
Caco-2 (H. sapiens) ATCC Cat# HTB-37
HEK 293 T (H. sapiens) ATCC Cat# CRL-3216; RRID:CVCL_0063
LS174T (H. sapiens) ATCC Cat# CL-188
LS174T human Flag-tagged LRH-1, 4-HT-inducible This study N/A
LS174T human Flag-tagged LRH-1 F342W/I416W, 4-HT-inducible This study N/A
LS174T murine LRH-1, 4-HT-inducible This study N/A
LS174T EGFP, 4-HT-inducible This study N/A
Bifidobacterium animalis subsp. lactis 420 (B420TM) Danisco Deutschland GmbH Sample #767, Batch 1102133655
Escherichia coli BL21 Previous hosting lab N/A
Escherichia coli Nissle 1917 (EcN) (Mutaflor) Ardeypharm N/A
Escherichia coli RosettaTM 2 (DE3) Novagen Cat# 71400
Enterobacter cloacae DSMZ DSM No.: 30054
Enterococcus faecalis DSMZ DSM No.: 20478
Klebsiella pneumoniae DSMZ DSM No.: 681
Pseudomonas aeruginosa PA01 DSMZ DSM No.: 22644
Proteus mirabilis DSMZ DSM No.: 4479
Symbioflor2 Symbiopharm N/A
Vibrio cholerae DSMZ DSM No.: 106276
Recombinant DNA
Plasmids Table 2
Antibodies
Mouse mAB anti-Flag M2 Sigma-Aldrich Cat# F1804
Mouse mAB anti-GFP Roche Cat# 11814460001
Mouse mAB anti-Tubulin Sigma-Aldrich Cat# T5168; RRID:AB_477579
Rabbit pAB anti-NR5A2 Sigma-Aldrich Cat# HPA005455
Oligonucleotides and other sequence-based reagents
Primers Table 1
Chemicals, enzymes and other reagents
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) Sigma-Aldrich Cat# M2128
4-hydroxytamoxifen Sigma-Aldrich Cat# H7904
Adenosine 5-triphosphate (ATP) Carl Roth Cat# K029.2
ammonium thiocyanate Sigma-Aldrich Cat# 1012130500
AMPure XP Beckman Coulter Cat# A63881
Cis-vaccenic acid Sigma-Aldrich Cat# V0384
Cis-vaccenic acid Cayman chemical Cat# 20023
Co-Enzyme A PJK Cat# 102211
cOmplete TM, EDTA-free Protease Inhibitor Cocktail Roche Cat# 04693132001
Compound 3d2 (3d2) ChemBridge Corp ID: 16728690
Direct Red 80 Sigma-Aldrich Cat# 365548
D-Luciferine Na salt (Luciferin) PJK Cat# 102132
DNase I (RNase-free) New England Biolabs Cat# M0303
Dulbecco’s Modified Eagle’s Medium Sigma-Aldrich Cat# D5796
Fetal Bovine Serum Sigma-Aldrich Cat# F7524
Gentamicin Sigma-Aldrich Cat # G1272
Hygromycin B InvivoGen Cat# ant-hg-1
Iscove’s Modified Dulbecco’s Medium Sigma-Aldrich Cat# I3390
Isopropyl β-D-1-thiogalactopyranoside (IPTG) Carl Roth Cat# 2316.3
JetPeiTM Polyplus Transfection Cat# 101000020
L-glutamine Sigma-Aldrich Cat# G7513
Luminol Fluka Cat# 09253
Oil Red O Sigma-Aldrich Cat# O0625
O-nitrophenyl-b-D-galactopyranoside (ONPG) Carl Roth Cat# CN.22
P-coumaric acid Sigma-Aldrich Cat# C-9008
PeqGOLD TriFastTM VWR Cat# 30–2030
Puromycin. 2HCl Enzo Life Sciences Cat# BML-GR312
SR1848 Sigma-Aldrich Cat# SML 1513
TEV-protease Sigma-Aldrich Cat# T4455
Tissue-Tek O.C.T Compound Sakura Cat# 4583
Trans- vaccenic acid Sigma-Aldrich Cat# V1131
TRIzolTM Reagent Invitrogen Cat# 15596026
Software
Bioanalyzer Smear Analysis tool Agilent Technologies N/A
MO.Affinity Analysis software NanoTemper Technologies version 2.2.4
NextSeq System Suite Illumina Inc v2.2.0
Prism GraphPad Software V7.0
QuantStudio Design & Analysis Software Applied Biosystems V1.5.2
R package “EnhancedVolcano” version 1.18.0
R package clusterProfiler version 4.8.3
R package DESeq2 version 1.40.2
R package pheatmap version 1.0.12
ZEN Software Carl Zeiss Microscopy V2.5 (blue edition)
Other
10 kDA Amicon Ultra tube Millipore Cat# UFC901024
High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814
High-fat diet with 45% calories from fat Safe lab SAFE 245HF, D12451
HiLoad 16/60 Superdex® 200 PG column Cytiva Cat# 28-9893-35
HisTrap HP column Cytiva Cat# 29-0510-21
Monolith NT Protein Labeling Kit GREEN-NHS NanoTemper Technologies Cat# MO-L002
Monolith NT.115 capillaries NanoTemper Technologies Cat# MO-K022
PD SpinTrap G-25 column Cytiva Cat# 28-9180-04
Pierce™ BCA Assay Kit Thermo Fisher Cat# 23225
PVDF membrane Roche Cat# 03010040001
QuikChange II XL Site-Directed Mutagenesis Kit Agilent Technologies Cat# 200521
Standard diet Safe lab SAFE A03
SV Total RNA Isolation System Promega Cat# Z3100
SYBR® Green Applied Biosystems Cat# 4385614
Triglyceride Quantification Kit Sigma-Aldrich Cat# MAK266
TruSeq® Stranded mRNA Sample Preparation Kit Illumina Inc Cat# 20020594
Ultra Sensitive Mouse Insulin ELISA Kit CrystalChem Cat# 90080
Venor®GeM Classic Minerva Biolabs Cat# 11-1050
3.5 KDa Slide-A-Lyzer MINI Dialysis Device ThermoFisher Scientific Cat# 88403
Bioanalyzer 2100 Agilent Technologies N/A
BioAnalyzer 4200 tapeStation Agilent Technologies N/A
Glucometer GlucoCheck N/A
Hydrax C500 cryostat Carl Zeiss N/A
Image Quant LAS4000 GE Healthcare N/A
Infinite® 200 PRO plate reader TECAN N/A
Monolith NT.115 instrument NanoTemper Technologies N/A
NextSeq500 System Illumina Inc N/A
Reflotron Plus Roche N/A
QuantStudio 3 Real-Time-PCR-System Applied Biosystems N/A
Zeiss Observer Z.1 microscope Carl Zeiss N/A