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. 2026 Jun 1;27(7):1490–1501. doi: 10.1038/s41590-026-02526-2

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© The Author(s) 2026, modified publication 2026

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a) The number and ratio of BL or DLBCL patients^{10,11,16,52–54} with and without TP53 alterations are shown according to RFX7 mutation status (related to Supplementary Table 3). Fisher’s exact test (two-sided); odds ratio (OR, 95% CI). b) The B cell compartment of 6–16 weeks old Cd19p53^wt/wtRfx7^wt/wt, Cd19p53^fl/fl, Cd19p53^fl/flRfx7^fl/fl, and Cd19p53^fl/flRfx7^fl/flMyc^fl/fl mice was analyzed at steady state. Graphs illustrate spleen cellularity, B cell (CD19⁺B220⁺), follicular B cell (CD21⁻CD23⁺), and marginal zone B cell (CD21⁺CD23⁻) percentages. Results represent mean±s.e.m. of n = 8 Cd19p53^wt/wtRfx7^wt/wt, n = 7 Cd19p53^fl/fl, n = 8 Cd19p53^fl/flRfx7^fl/fl, and n = 5 Cd19p53^fl/flRfx7^fl/flMyc^fl/fl mice and are a pool of two independent experiments. Statistical comparisons were performed using one-way ANOVA corrected for multiple testing using the Dunnett method; only significant results between Cd19p53^fl/flRfx7^fl/fl mice and the other groups are shown. c) Survival curves of n = 11 Cd19p53^fl/fl, n = 8 Cd19p53^fl/flRfx7^fl/fl, n = 8 Cd19p53^fl/flRfx7^fl/flMyc^fl/wt, n = 9 Cd19p53^fl/flRfx7^fl/flMyc^fl/fl, and n = 7 Cd19p53^fl/flRfx7^fl/flAicda^−/− mice. Mice were sacrificed at the onset of symptoms or at the end of the experiment at 300 days. Statistical comparisons between the experimental groups were performed using Mantel Cox test; only significant comparisons are shown. d) Immunoblot analysis showing the expression of Rfx7 and p53 in lysates of untreated or CpG-stimulated (18 h) B cells enriched from spleens of Cd19p53^wt/wtRfx7^wt/wt, Cd19Rfx7^fl/fl, Cd19p53^fl/fl, and Cd19p53^fl/flRfx7^fl/fl mice. β-actin was used as loading control and data are representative of two independent experiments. e) Heatmap of a selection of genes (Tob1, Tsc22d1, Mxd4, Ddit4, Trp53, Psrc1, and Serpine2) from RNA-seq analysis presented in Fig. 2d is shown. f) Sorted splenic FO B cells (CD19⁺, B220⁺ and CD23⁺) of 6 to 14-week-old mice of indicated genotypes were stimulated with CpG (18 h). Graphs show mRNA levels of Mxd4, Tob1, Tsc22d1, Ddit4, Serpine2, and Psrc1 assessed by RT-qPCR (values normalized to Hprt and 18 s; results are pool of two experiments, one control from each experiment was set to 1). Results represent mean ± s.e.m. of n = 4 Cd19Rfx7^wt/wt, n = 5 Cd19Rfx7^fl/fl, n = 6 Cd19p53^fl/fl, and n = 6 Cd19p53^fl/flRfx7^fl/fl mice. Statistical comparisons were performed using two-way ANOVA corrected for multiple testing using Tukey method; only significant comparisons are shown. g) Graphs show percentage of blasting B cells of indicated genotypes, 18 h post-stimulation with CpG, and the representative flow cytometry plots thereof (results represent mean±s.e.m. of n = 3 mice/group). Statistical comparisons were performed using two-way ANOVA corrected for multiple testing using Tukey method; only significant comparisons are shown. h) The B cell compartment of 6–14 weeks old Cd19p53^wt/wtRfx7^wt/wt and Cd19p53^fl/flRfx7^fl/flAicda^−/− mice was analyzed at steady state. Graphs illustrate spleen cellularity, B cell (CD19⁺B220⁺), follicular B cell (CD21⁻CD23⁺), and marginal zone B cell (CD21⁺CD23⁻) percentages. Results represent mean±s.e.m. of n = 4 Cd19p53^wt/wtRfx7^wt/wt and n = 3 Cd19p53^fl/flRfx7^fl/flAicda^−/− mice. Statistical comparisons were performed using unpaired student’s t-test, two-tailed.

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