FIG. 3.
Protein synthesis in infected L929 cells. Cells were mock infected (A) or infected with wild-type mengovirus (A), vM16ΔL(12-52) (B), vM16T47A (C), vM16E50A (D), vM16insG49 (E), or vM16Δ2A (F) as indicated at an MOI of 50 TCID50/cell. At the time points indicated above the lanes (in hours postinfection), cells were pulse-labeled with [35S]methionine for 30 min. Cells were lysed and directly analyzed by SDS-PAGE on 12.5% polyacrylamide gels (upper panels) or used for immunoprecipitation of ferritin (lower panels). Viral proteins and heavy (H) and light (L) ferritin chains are indicated.