FIGURE 2.
Axl deficiency alters the metabolic and oxidative profile of peritoneal macrophages. (A) Nitrite production by peritoneal macrophages (PMs) from wild‐type (WT) and Axl‐deficient (Axl−/−) mice under unstimulated conditions after 24 and 48 h. Data are presented as mean ± SD compiled from three independent experiments. (B) Lactate accumulation in culture supernatants under unstimulated conditions after 24 and 48 h. (C) Lactate levels in PM culture supernatants after 24 h of LPS stimulation. (D) Lactate dehydrogenase (LDH) levels in culture supernatants following 24 h of LPS stimulation, used to assess cell viability. (E) Reactive oxygen species (ROS) production by PMs over 60 min under unstimulated conditions, measured using dihydroethidium (DHE). (F) ROS production by PMs over 60 min following LPS stimulation, measured using DHE. (G) Percentage of MitoSOX‐positive PMs. (H) Mean fluorescence intensity (MFI) of MitoSOX Red within the MitoSOX‐positive PM population, reflecting mitochondrial ROS levels per cell. (B–G) Data are presented as mean ± SD from three independent experiments. Statistical significance was determined using Student's t‐test for panels (E–H) and one‐way ANOVA followed by Tukey's multiple comparisons test for panels A–D (p < 0.05; *p < 0.01; **p < 0.001; ***p < 0.0001).