FIG. 3.
(A) CT values for each primer pair in the KSHV array. Closed circles indicate the CT values if poly(A) mRNA without RT was used (RTneg); open circles indicate the CT values if the mRNA was subjected to RT (RTpos). The vertical axis indicates the CT value, and the horizontal axis indicates the percentile of primers. (B) Frequency histogram of the dCT difference between RTpos and RTneg reactions for each primer pair. Superimposed is the expected normal distribution. The relative frequency is shown on the vertical, and the dCT is shown on the horizontal axis. (C) SD (on the horizontal scale) and mean (n = 3) CT values for a group of amplicons. The dotted line indicates the mean of means.