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Cholesterol depletion does not significantly affect the surface expression of CD4 and CXCR4 receptors. Untreated PM1 T cells or cells treated with 10 mM MβCD were stained for 30 min on ice with FITC-conjugated monoclonal antibodies against CD4 (13B8.2) or CXCR4 (12G5) and subsequently analyzed by flow cytometry as described in Materials and Methods.
