Fig. 5.
Loss of P-TBI induced microglia activity in C3−/−/CD14−/− mice. A, B Representation of analyzed brain regions of wildtype mice post sham and P-TBI, depicting a brainwide induction of pS6 in IBA1 + microglia post P-TBI. C–H Immunofluorescence staining and imaging of pS6 and IBA1 revealed increase of pS6 intensity in microglia in both ipsi- and contralateral cortex (WT sham vs. WT P-TBI ipsi: p = 0.003; WT sham vs. WT P-TBI contra: p = 0.003), hippocampus (WT sham vs. WT P-TBI ipsi: p = 0.009; WT sham vs. WT P-TBI contra: p = 0.007; despite the non significant omnibus analysis: p = 0.108) and striatum (WT sham vs. WT P-TBI ipsi: p = 0.002; WT sham vs. WT P-TBI contra: p = 0.023). No increase of pS6 intensity was observed in bilateral cortex, hippocampus or striatum for C3−/−/CD14−/− mice. Data shown as dot plots with single microglia depicted as black dots and single mice as coloured dots (cyan). Scale bar overview: 200 μm; insert: 10 μm. Group size: N = 3–4. Exact p-values are reported, significances are shown in bold