TABLE 1.
Binding of antienvelope MAbs to parental and FS envelopes
| Envelopea | Mean ± SEM % binding of MAbb:
|
||||
|---|---|---|---|---|---|
| b12 (CD4bs) | 2G12 (complex) | 697D (V2) | 694/98D (V3) | 246D (gp41) | |
| 8x | 100 | 100 | 100 | 100 | 100 |
| HXB | 54 ± 8 | 53 ± 7 | 95 ± 2 | 96 ± 16 | 41 ± 11 |
| HXB/FS | 117 ± 11 | 105 ± 10 | 102 ± 5 | 108 ± 8 | 107 ± 21 |
| UGO-24 | 17 ± 6 | 42 ± 5 | 98 ± 3 | 73 ± 11 | 45 ± 15 |
| UGO-24/FS | 64 ± 5 | 100 ± 14 | 104 ± 7 | 71 ± 17 | 112 ± 13 |
| JRFL | 65 ± 5 | 57 ± 5 | 97 ± 2 | 99 ± 16 | 40 ± 13 |
| JRFL/FS | 111 ± 6 | 147 ± 12 | 104 ± 3 | 109 ± 21 | 76 ± 20 |
| ADA | 49 ± 4 | 63 ± 8 | 96 ± 3 | 90 ± 11 | 34 ± 11 |
| ADA/FS | 70 ± 4 | 44 ± 13 | 96 ± 3 | 95 ± 28 | 45 ± 17 |
| ADA-CD4i | 21 ± 10 | 54 ± 18 | 92 ± 2 | 89 ± 19 | 30 ± 7 |
| ADA-CD4i/FS | 48 ± 13 | 123 ± 35 | 90 ± 2 | 92 ± 14 | 83 ± 12 |
| 89.6 | 65 ± 3 | 66 ± 21 | 95 ± 6 | 99 ± 20 | 34 ± 10 |
| 89.6/FS | 116 ± 13 | 153 ± 33 | 123 ± 13 | 116 ± 22 | 112 ± 15 |
a
/FS, containing FS.
b
Material in parentheses indicates regions for which MAbs are specific. bs, binding site. Significantly different data are shown in bold. The data are normalized to the amount of antibody bound to the 8× Env. The data are representative of three experiments done in triplicate.