Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat monoclonal anti-FKBP51 | R&D Systems | Cat#MAB4094; RRID:AB_1964585 |
| Rat polyclonal anti-FKBP51 | R&D Systems | Cat#AF4094; RRID:AB_2103136 |
| Rabbit monoclonal anti-FKBP51 | Cell Signaling Technology | Cat#12210S; RRID:AB_2797846 |
| Rabbit polyclonal anti-FKBP52 | Cell Signaling Technology | Cat#11826S; RRID:AB_2797737 |
| Rat monoclonal anti-Hsp90 | Enzo Life Sciences | Cat#ADI-SPA-835; RRID:AB_2039281 |
| Rabbit polyclonal anti-V5 Tag | Bethyl Laboratories, Inc | Cat#A190120A; RRID:AB_67586 |
| Mouse monoclonal anti-Lamin | Proteintech | Cat#66095-1-Ig; RRID:AB_11232208 |
| Mouse monoclonal anti-Alpha Tubulin | Proteintech | Cat#66031-1-Ig; RRID:AB_11042766 |
| Rabbit polcyclonal anti-PHB1 | Cell Signaling Technology | Cat#2426S; RRID:AB_823689 |
| Rabbit polyclonal anti-ALDH3A1 | Proteintech | Cat#15578-1-AP, RRID:AB_2273745 |
| Mouse monoclonal anti-β Actin | Proteintech | Cat#66009-1-Ig; RRID:AB_2687938 |
| Rabbit polyclonal anti-β Actin | Proteintech | Cat#0536-1-AP; RRID:AB_10700003 |
| HRP-conjugated anti-Mouse IgG | Southern Biotech | Cat#030-05; RRID:AB_2619742 |
| HRP-conjugated anti-Rabbit IgG | Southern Biotech | Cat#4010-05; RRID:AB_2632593 |
| HRP-conjugated anti-Rat IgG | Southern Biotech | Cat#3010-05; RRID:AB_2795801 |
| Alexa Fluor 549 anti-Goat (Donkey) | Invitrogen | Cat#A32758; RRID:AB_2762828 |
| Alexa Fluor 647 anti-Rabbit (Donkey) | Invitrogen | Cat#A32795; RRID:AB_2762835 |
| Mouse Anti-V5-tag Magnetic Beads | MBL International Corporation | Cat#M167-11; RRID:AB_3716385 |
| Bacterial and virus strains | ||
| DH5α Competent cells | Invitrogen | Cat#18265017 |
| Critical commercial assays | ||
| Pierce BCA protein assay kit | ThermoFisher Scientific | Cat#PI-23225 |
| Mitochondria isolation kit for cultured cells | Abcam | Cat#ab110171 |
| Seahorse XFe96/XF Pro FluxPak | Agilent Technologies | Cat#103793100 |
| Deposited data | ||
| Mass Spectrometry data and related related MS-DAP QC report | This paper | MassIVE: MSV000099315 |
| Experimental models: Cell lines | ||
| HeLa | ATCC | Cat# CCL-2; RRID:CVCL_0030 |
| Recombinant DNA | ||
| pCDNA3 V5-TurboID-NES | Addgene | 107169; RRID:Addgene_107169 |
| pCDNA3 V5-FKBP51-GS-TurboID-NES | Genscript, this paper | N/A |
| pCDNA3 V5-TurboID-GS-FKBP51-NES | Genscript, this paper | N/A |
| pCDNA3 V5-FKBP52-TurboID-NES | Genscript, this paper | N/A |
| pCMV6 empty vector | Jinwal et al.12 | N/A |
| pCMV6 FKBP51 | Jinwal et al.12 | N/A |
| pCMV6 FKBP52 | Jinwal et al.12 | N/A |
| TOMM20-C-GFPSpark | Sino Biological | Cat#HG15094-ACG |
| Software and algorithms | ||
| Zen 3.3 | Zeiss | v3.3.89.0000 |
| DIA-NN | Demichev et al.37 | v1.8.1 |
| MS-DAP | Koopmans et al. 38 | v1.2.2 |
| STRING | Szklarczyk et al. 39 | v12.0 |
| Metascape | Zhou et al. 40 | v3.5.20260201 |
| Cytoscape | Shannon et al 41 | v3.10.4 |
| String App for Cytoscape | Doncheva et al. 42 | v2.2.0 |
| ShinyGo | Ge et al. 43 | v0.85 |
| R version 4.5.2 | The R Project for Statistical Computing | v4.5.2 |
| ComplexHeatmap R package | Gu et al. 44 | v2.26.0 |
| Circlize R package | Gu et al. 45 | v0.4.17 |
| GraphPad Prism | GraphPad | v11.0.0 |