Figure 4.
RHO-1 enhances acetylcholine release by DGK-1-dependent and -independent mechanisms. Presynaptic expression (from the unc-17 promoter, N:C3 transferase or N:C3T) of C3 transferase reduces the locomotion rate (A) and aldicarb sensitivity (B) of dgk-1(sy428) mutant animals, although not to the same level as animals with presynaptic expression of C3 transferase in nonmutant animals, indicating that some but not all RHO-1 enhancement of ACh release occurs via inhibition of DGK-1. Error bars indicate standard error of the mean. Statistical analysis was performed using a two-tailed t-test, and two asterisks (**) indicates significance (p < 0.001).