TABLE 2.
Relative survival of radA and rec mutant strains to DNA-damaging agentsa
| Strain | Genotype | Relative survivalb to:
|
||||
|---|---|---|---|---|---|---|
| MMS | MMC | Phleomycin | H2O2 | HU | ||
| radA single mutants | ||||||
| AB1157 | Wild type | 1 | 1 | 1 | 1 | 1 |
| SR776 | radA100 | 2.1 × 10−2 | 3.5 × 10−1 | 4.6 | 1.1 | 3.8 × 10−4 |
| STL5280 | radA1::kan | 6.6 × 10−2 | 7.5 × 10−1 | 2.2 × 10−2 | 1.1 | 1.4 |
| STL6430 | radAΔ3 | 1.3 × 10−1 | 4.0 × 10−1 | 9.5 × 10−2 | 9.6 × 10−1 | 2.9 × 10−1 |
| Holliday junction-processing mutants | ||||||
| N4452 | recG | 1.9 × 10−4 | 1.1 × 10−1 | 2.0 × 10−1 | 4.1 × 10−1 | 2.1 × 10−4 |
| STL6588 | recG radA1 | 4.5 × 10−7 | 1.8 × 10−4 | 4.4 × 10−4 | 5.9 × 10−3 | 3.9 × 10−5 |
| N2096 | ruvA | 6.4 × 10−5 | 8.6 × 10−6 | 7.1 × 10−4 | 6.7 × 10−2 | 5.1 × 10−5 |
| STL5037 | ruvA radA1 | 7.6 × 10−6 | 3.5 × 10−7 | 1.9 × 10−4 | 5.3 × 10−3 | 1.9 × 10−5 |
| CS140 | ruvC | 2.8 × 10−6 | 2.6 × 10−6 | 6.9 × 10−4 | 7.3 × 10−2 | 1.2 × 10−4 |
| STL5046 | ruvC radA1 | 1.1 × 10−5 | 7.4 × 10−7 | 1.8 × 10−4 | 1.1 × 10−2 | 1.4 × 10−4 |
| Mutants in other recombination functions | ||||||
| JC12123 | recJ | 3.2 × 10−2 | 1.8 × 10−1 | 1.8 | 4.2 × 10−1 | 6.6 × 10−1 |
| STL5042 | recJ radA1 | 6.8 × 10−3 | 2.2 × 10−1 | 2.7 × 10−1 | 7.3 × 10−1 | 4.1 × 10−1 |
| STL1528 | recQ | 4.4 × 10−1 | 5.3 × 10−1 | 3.2 × 10−3 | 4.8 × 10−1 | 1.4 |
| STL5048 | recQ radA1 | 2.8 × 10−2 | 4.4 × 10−1 | 2.8 × 10−4 | 9.8 × 10−1 | 1.9 |
| N2101 | recB | 2.7 × 10−2 | 4.9 × 10−3 | 5.3 × 10−3 | 3.1 × 10−2 | 9.9 × 10−5 |
| STL5480 | recB radA1 | 2.5 × 10−2 | 2.7 × 10−3 | 2.9 × 10−4 | 1.0 × 10−1 | 7.3 × 10−2 |
| JC10287 | recA | 1.9 × 10−5 | 1.2 × 10−5 | 1.6 × 10−3 | 1.7 × 10−3 | 1.1 × 10−3 |
| STL4799 | recA radA1 | 1.7 × 10−6 | 3.0 × 10−6 | 2.9 × 10−4 | 1.4 × 10−2 | 6.0 × 10−1 |
a
Determinations were performed for eight independent cultures on at least two different days, with standard errors of the values in all cases less than 70% of the reported value. Allele designations for all genetic markers other than radA are not shown but can be found in Table 1.
b
Survival of the mutant strains is expressed relative to that of AB1157, which was given a value of 1.