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The presence or absence of ppGpp does not alter the DksA accumulation. Cultures of strains A to D were grown in LB medium, and in each case six samples were taken representing the growth transition to stationary phase as in Fig. 5. The DksA content was analyzed by Western blots. (A) CF1648 = MG1655 (wild type); (B) CF1648/pdksA; (C) CF1693 ΔrelA ΔspoT ppGpp0; and (D) CF1693/pdksA. The presence of the pdksA plasmid is indicated by a plus at the top of sample series B and D.
