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Purification and gel filtration of SSO0287. (A) Coomassie blue-stained SDS-PAGE gel of Strep tag-specific affinity chromatography fractions of the cytoplasm of SSO0287 transformants. St, starting material; FT, flowthrough; E, elution fraction. (B) Gel filtration of purified SSO0287. Traces were recorded at 280 nm (black line) and 410 nm (dashed line).
