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. 2006 Jan;72(1):102–111. doi: 10.1128/AEM.72.1.102-111.2006

FIG. 4.

FIG. 4.

Western analysis of FlaI expression. S. solfataricus cells transformed with pSVA6 and control PH1-16 cells were grown for 2 days at 88°C. Samples were taken at the beginning of the temperature shift from 78 to 88°C and after 1 and 2 days of growth at 88°C. Membranes were isolated from the cells and were analyzed by SDS-PAGE and immunoblotting using FlaI-specific antibodies. The arrow indicates the detected expression product.