FIG. 2.
(A) Comparison of nodY-lacZ expression in B. japonicum USDA110 or JNWS21 (nwsB) mutant at a low or high population density. B. japonicum cells were grown to an OD600 of 0.1 or 1.8, and the ability of 1 μM genistein to induce the nodY-lacZ expression was determined after 12 h of incubation. (B) Effect of CM on nodY-lacZ expression in USDA110 or JNWS21 (nwsB mutant). B. japonicum cultures (OD600 = 0.1) were induced for 5 h with CM. The CM was obtained from B. japonicum cultures grown to an OD600 of 2.0. The results represent the means of three separate experiments ± the standard deviation. Under the conditions used, an OD600 unit corresponded to ca. 109 cells/ml.