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. Author manuscript; available in PMC: 2026 Aug 18.
Published in final edited form as: Structure. 2026 Apr 17;34(6):977–987.e2. doi: 10.1016/j.str.2026.03.005

Figure 1. SXT imaging and calculation of ISG parameters.

Figure 1.

(A) SXT full-rotation projection images, which are combined to create a 3D image stack. (B) The LAC values in the SXT data correspond to biochemical content and are necessary for quantifying ISG maturities. (C) Blender rendering of a segmented INS-1E cell. ISG, nucleus, and mitochondria masks are shown. (D) Zoomed in image of an SXT orthoslice and LAC histogram of a selected ISG. Analysis at this resolution enables the creation of individual granule LAC statistics. (E) ISGs overlaid on the EDT of the plasma membrane. ISGs are displayed in white and PM displayed as a dashed white line. (F) Mitochondria mask inverse EDT with overlaid organelles. ISGs are again displayed in white, with PM displayed as a dashed white line. Mitochondria are colored black.