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Production of IL-2 and IFN-γ by pcDNA3-transfected and pcDNA3-GILZ-transfected clones
| Transfecting plasmid | Mean cytokine concn ± SD
|
|||
|---|---|---|---|---|
| Control cells
|
Anti-CD3 antibody-treated cells
|
|||
| IL-2 (pg/ml) | IFN-γ (ng/ml) | IL-2 (pg/ml) | IFN-γ (ng/ml) | |
| pcDNA3a | -b | - | 745 ± 10 | 8 ± 0.5 |
| pcDNA3-GILZ | - | - | 20 ± 1.5 | 12 ± 1 |
3DO clones containing pcDNA3 or pcDNA3-GILZ were treated for 18 h with adherent anti-CD3 MAb (1 μg/ml). Supernatants were harvested and used in an ELISA.
-, None detected.
