TABLE 1.
Strains used in this study
| Strain | Genotype |
|---|---|
| JY333 | h−ade6-M216 leu1 |
| JY450 | h90ade6-M216 leu1 |
| JX222 | h−ade6-M216 leu1 ura4-D18 cgs1::ura4+ |
| JX231 | h90ade6-M216 leu1 ura4-D18 rst2::ura4+ |
| JX233 | h−ade6-M216 leu1 ura4-D18 rst2::ura4+ |
| JX261 | h−ade6-M216 leu1 ura4-D18 cgs1::ura4+rst2::ura4+ |
| JX282 | h−ade6-M216 leu1 ura4-D18 pka1::ura4+rst2::ura4+ |
| JX384 | h−ade6-M216 leu1 ura4-D18 pka1::ura4+ |
| JW352 | h−ade6-M216 leu1 ura4-D18 rst2-GFP(S65T)≪ura4+a |
| JW355 | h−ade6-M216 leu1 ura4-D18 rst2-GFP(S65T)≪ura4+cyr1::ura4+ |
| JW359 | h−ade6-M216 leu1 ura4-D18 rst2-GFP(S65T)≪ura4+cgs1::ura4+ |
| JW363 | h−ade6-M216 leu1 ura4-D18 rst2-GFP(S65T)≪ura4+pka1::ura4+ |
| JW367 | h−ade6-M216 leu1 ura4-D18 rst2-3HA≪ura4+ |
| JW373 | h−ade6-M216 leu1 rst2-M3 |
| JW374 | h−ade6-M216 leu1 ura4-D18 cgs1::ura4+rst2-M3 |
| JW377 | h−ade6-M216 leu1 ura4-D18 pka1::ura4+rst2-M3 |
| JW487 | h90ade6-M216 leu1 ura4-D18 cgs1::ura4+rst2::ura4+ |
a
The expression rst2≪ura4+ indicates that ura4+ is inserted in the vicinity of rst2 on the chromosome.