FIG. 10.
Activation of caspase 3 and cleavage of PKCδ in cells overexpressing PKCδ and PKCδ5. C6 cells overexpressing control vector (CV), PKCδ, or PKCδ5 were treated with etoposide (50 μM) for 24 h, and the activation of caspase 3 (A and B) and cleavage of PKCδ (C) were determined. Cells were harvested and subjected to SDS-PAGE and Western blot analysis. The membranes were probed with active caspase 3 antibody (A) or with anti-PKCɛ that recognizes the ɛ tag which is located in the catalytic domain of the PKCδ constructs (C). The results represent one of three separate experiments which yielded similar results. The activity of caspase 3 was measured using the Caspase 3 Cellular Activity Assay Kit as described in Materials and Methods (B). Caspase 3 activity was calculated and expressed as picomoles/minute/microgram of protein, and the percent of maximal effect was determined. The results represent the means ± SE of three experiments.