FIG. 6.
Role for NrbI in spine development. Cultured rat hippocampal neurons were cultured for 7 DIV prior to transfection with the plasmid expressing either GFP or WT GFP-NrbI (1-1095). The following day (DIV 8) extensive filopodia were induced only in the NrbI-expressing neurons, with GFP-NrbI localized within these structures. Following DIV 17, spine development was observed in all neurons with GFP distributed throughout the cell. In contrast, NrbI overexpression resulted in the development of numerous and much larger spines, with the GFP-NrbI being highly concentrated in these structures (bars, 5 μm).