FIG. 3.
Analysis of DNA isolated from embryos of +/− intercrosses. (A) PCR of yolk sac DNA from 9.5-day-old embryos with PCR primers P1 and either P2 or P3 amplified a 916-bp wild-type fragment or 1,306-bp targeted fragment. L, 100-bp DNA Ladder Plus (MBI Fermentas). (B) Southern blot analysis of yolk sac DNA digested with BamHI and hybridized with the flanking probe E2/161. The 5-kb band indicates transmission of the wild-type (wt) allele, and the 11-kb band indicates transmission of the targeted (KO) allele.