Figure EV6. Mitochondrial activity in cyst cells does not depend on autonomous pyruvate consumption.
(A) Airyscan image of a testis in which GFP (cyan) was expressed with tj-Gal4 to identify cyst cells and labelled with the mitochondrial membrane potential-sensitive dye TMRM (yellow). The hub is indicated with an asterisk. Scale bar: 20 μm. (B) Graph showing TMRM mean intensity in cyst cells in control testes and testes in which Pfk or Pyk were knocked down. Significance was determined by Kruskal– Wallis and Dunn’s multiple comparisons tests. (C) Graph showing the number of Lysotracker-positive cysts in control testes and testes in which Mpc1 was knocked down in cyst cells. Significance was determined by Kruskal–Wallis and Dunn’s multiple comparisons tests. All graphs: N values are shown in brackets and refer to the number of testes analysed. Source data are available online for this figure.
