FIG. 3.
α-Satellite DNA fragments with CENP-B boxes were concentrated by CHIP using anti-CENP-A, anti-CENP-B, or anti-CENP-C antibodies. Before IP, clones prepared from the solubilized bulk chromatin before immunoprecipitation. IP, DNA fragments immunoprecipitated by anti-CENP-A (αCA), anti-CENP-B (αCB), or anti-CENP-C (αCC) antibodies were cloned into T vector and sequenced. More than 80% of the cloned fragments were longer than 500 bp. The black bars show the number of clones with α-satellite DNA that contained the CENP-B box. The open bars show the number of clones that were unique and contained no other repetitive sequence, not even α-satellite DNA without the CENP-B box.