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Confocal microscopy analysis of cytochrome c immunostaining in control and Hsp27-expressing L929 cells exposed or not to staurosporine. Control L929 cells (C2) and L929-Hsp27 cells were either kept untreated (NT) or treated for 3 h with 1 μM staurosporine (3 h ST). Cells were prepared for immunostaining analysis using anti-cytochrome c antibody and observed under a confocal fluorescence microscope as described in Materials and Methods.
