FIG. 2.
Purity and integrity of PpAMA1's. SDS-PAGE on 4-to-20% gradient Tris-glycine polyacrylamide gels was performed on serial fourfold dilutions of purified PpAMA1 FVO (A and B) and PpAMA1 3D7 (C and D). Quantities loaded were, from left to right, 12.5, 3.1, 0.8, and 0.2 μg. Electrophoresis was performed either under nonreducing conditions (A and C) or with 5% (vol/vol) β-mercaptoethanol (B and D). Scanning laser densitometry showed yields of 96.9% (nonreduced) or 81.9% (reduced) full-length PpAMA1 FVO and 97.6% (nonreduced) or 48.4% (reduced) full-length PpAMA1 3D7. Single asterisks indicate bands whose N-terminal sequence was identified by Edman degradation as Y1VQNYWEHPYQKSDVYHPIN (SynFVO) or Y1VQNYWEHPYQNSDVYRPIN (Syn3D7); double asterisks indicate bands whose N-terminal sequence was S355AFLPTGAFKADRYKSH.