Table 1.
Twenty-five genes that were most significantly (t test, P < 0.05) up- or down-regulated between the indicated groups of placentas
| ES cell list | ES/Con | Cumulus list | Cum/Con |
|---|---|---|---|
| Elevated in clones | |||
| Ada adenosine deaminase | 3.34∧ | Prlpi prolactin-like protein I | 3.34∧ |
| Ncam neural cell adhesion | 3.20∧ | Ada adenosine deaminase | 2.43∧ |
| Car2 carbonic anhydrase 2 | 2.82∧ | Pthr parathyroid hormone r | 2.16 |
| Ada adenosine deaminase | 2.67∧ | Ada adenosine deaminase | 2.14∧ |
| Chemokine (C-C) receptor | 2.58∧ | Jak1 Janus kinase 1 | 2.11∧ |
| Prlpg prolactin-like protein | 2.47 | Car2 carbonic anhydrase 2 | 2.05 |
| Onzin | 2.26∧ | Cd83 CD83 antigen | 2.03∧ |
| Mmp15 matrix metalloprot | 2.23∧ | Mmp15 matrix metalloprot | 2.02∧ |
| Gpc1 glypican 1 | 2.15∧ | Sparc secreted glycoprot | 2.01 |
| 2610042L04Rik RIKEN cDNA | 2.14∧ | Fbln1 fibulin 1 | 1.94∧ |
| Lysozyme M | 2.11 | Prlpg prolactin-like protein | 1.94∧ |
| Prkcl protein kinase C | 2.07 | Tyrosine phosphatase LAR | 1.92∧ |
| Prlpi prolactin-like protein | 2.05∧ | Cbx4 chromobox homolog | 1.88∧ |
| Oxidized LDL receptor | 2.04 | 1200008D14Rik RIKEN cDNA | 1.86 |
| Pl1 placental lactogen 1 | 2.04∧ | Hdac6 histone deacetylase | 1.85∧ |
| Fbln1 fibulin 1 | 2.01∧ | Chemokine (C-C) receptor 1 | 1.82∧ |
| Cd83 CD83 antigen | 2.00∧ | Ctss cathepsin S | 1.80∧ |
| Col15a1 Procollagen,XV | 1.96 | Ncam neural cell adhesion | 1.80∧ |
| Ctss cathepsin S | 1.90 | Pl1 placental lactogen 1 | 1.79 |
| Glk galactokinase | 1.86∧ | Adcy7 adenylate cyclase 7 | 1.78∧ |
| Dtx1 deltex 1 homolog | 1.86∧ | Lysozyme M | 1.75 |
| Reduced in clones | |||
| Eln elastin | 0.36∧ | Eln elastin | 0.30∧ |
| 1600025H15Rik RIKEN cDNA | 0.38∧ | 1600025H15Rik RIKEN cDNA | 0.34∧ |
| 5033414D02Rik RIKEN cDNA | 0.43∧ | Vnn1 vanin 1 | 0.37∧ |
| EST: AW121826 | 0.45∧ | EST: AW121826 | 0.39∧ |
| Hsd11b1 dehydrogenase | 0.46∧ | Hsd11b1 hydroxysteroid 11 | 0.42∧ |
| Slc1a4 solute carrier family 1 | 0.47∧ | 5033414D02Rik RIKEN cDNA | 0.42∧ |
| Folr1 Folate receptor 1 | 0.47∧ | 1200011C15Rik RIKEN cDNA | 0.44∧ |
| 1200011C15Rik RIKEN cDNA | 0.48∧ | F2rl1 coagulation factor II | 0.44∧ |
| Ccr4 carbon catabolite hom | 0.48∧ | Folr1 folate receptor 1 | 0.44∧ |
| Na dep vitamine transport | 0.48∧ | Carbonic anhydrase IV | 0.44∧ |
| Eng endoglin | 0.48 | Col18a1 procollagen XVIII | 0.47∧ |
| AA589632 EST | 0.49∧ | AI785303 EST | 0.47∧ |
| 2610019F03Rik RIKEN cDNA | 0.51∧ | Ephrin B1 | 0.47∧ |
| Grb10 growth factor rbp | 0.52∧ | Grb10 growth factor rbp | 0.48∧ |
| AI785303 EST | 0.53∧ | Slc1a4 solute carrier family 1 | 0.50∧ |
| Vnn1 vanin 1 | 0.53∧ | ESTs, highly similar to SL56 | 0.50∧ |
| Trfr transferrin receptor | 0.53∧ | 1-Cys peroxiredoxin prot 2 | 0.51∧ |
| 1810004P07Rik RIKEN cDNA | 0.53 | 2610019F03Rik RIKEN cDNA | 0.52∧ |
| MHC class III region | 0.55∧ | Vegf | 0.52∧ |
| Carbonic anhydrase IV | 0.55∧ | Perp-pending, Pmp22related | 0.52 |
| Mpp1 membrane protein | 0.55∧ | MHC class III region | 0.53∧ |
All genes shown have a coefficient of variation of less than 25% for controls. Gene names correspond to their Unigene identification where possible, and otherwise their Affymetrix identifier. Genes are displayed from the greatest to least fold-change and the specified NT/control expression ratio is indicated. Genes that changed in both donor cell data sets appear in bold. Genes showing a consistent expression pattern when data from the different array versions were assessed independently are indicated as follows. Genes with a NT/control expression ratio of greater than 1.5 or less than 0.65 for both sets of experiments, for elevated and reduced expression respectively, are indicated by an ∧ next to the expression ratio. The complete list of changed genes is in Table 5.