. 2004 May;86(5):2910–2922. doi: 10.1016/S0006-3495(04)74342-8

TABLE 1.

Miscibility transition temperatures (T_mix) and lower transition temperatures (T_low) for membranes of DPPC, DOPC, and cholesterol using different methods

	T_mix			T_low
	Microscopy	²H-NMR	¹H-MAS NMR	Microscopy	²H-NMR
2:1 DOPC/DPPC-d62 + 30% Chol	24 ± 1°C	24 ± 2°C		12 ± 2°C	12 ± 2°C
2:1 DOPC/DPPC + 30% Chol-d1	29 ± 1°C				15 ± 5°C
2:1 DOPC/DPPC + 30% Chol	29 ± 1°C
1:1 DOPC/DPPC-d62 + 30% Chol	29 ± 1°C	29 ± 1°C		21 ± 2°C	21 ± 1°C
1:1 DOPC/DPPC + 30% Chol-d1	32 ± 1°C				26 ± 1°C
1:1 DOPC/DPPC + 30% Chol	32 ± 1°C		32 ± 1°C	21 ± 3°C
1:2 DOPC/DPPC-d62 + 30% Chol	33 ± 1°C	33 ± 1°C			28 ± 3°C	29 ± 1°C
1:2 DOPC/DPPC + 30% Chol-d1	36 ± 1°C					>30°C
1:2 DOPC/DPPC + 30% Chol	36 ± 1°C

²H-NMR experiments used deuterated lipid samples (either DPPC-d62 or Chol-d1), ¹H-MAS NMR measurements used only protonated lipids, and fluorescent microscopy measurements were conducted on both protonated and deuterated lipid mixtures. In microscopy experiments, T_mix marks the onset of μm-scale demixing in GUVs. In ¹H-MAS NMR experiments, significant spectral broadening initiates at T_mix and using ²H-NMR, a plateau begins in the first moment of DPPC-d62 spectra. In ²H-NMR, superposition of L_o and L_α phases are first observed at T_low. Additional evidence for a transition at T_low is seen as saturation in the surface fraction of dark phase in microscopy experiments.