Figure 4.
Coimmunoprecipitation of epitope-tagged CgA and SgIII constructs from the AtT-20 cell lysates. (A) AtT-20 cell lysates transfected with combinations of CgA 1–448-HA and SgIII 1–471-FLAG (lane 1), CgA 1–448-HA and SgIII Δ(187–373)-FLAG (lane 2), CgA Δ(41–109)-HA and SgIII 1–471-FLAG (lane 3), or CgA Δ(41–109)-HA and SgIII Δ(187–373)-FLAG (lane 4) were precipitated with mouse mAb to FLAG (A, top) and rat mAb to HA (A, bottom). Immunoprecipitates were run on an SDS-PAGE for immunoblotting with anti-HA (top) and anti-FLAG (bottom) antibodies. (B) One-fifth of the cell lysates and the corresponding media were immunoblotted with HA and FLAG antibodies to confirm their expression in AtT-20 cells.