Fig. 4.
Opn-/- T cells fail to elicit Th1 response. (A) Levels of IFN-γ and IL-10 were measured for day 4 cell culture supernatants. Cells of indicated types were plated as 2.5 × 105 cells per ml in precoated wells with 2.5 μg/ml of anti-CD3 Ab. Values show ratios of IFN-γ/IL-10 of ELISA. (B) EAE was induced in B6(N16)2D2+ mice (Opn-WT/HZ, n = 19, •; Opn deficient, n = 14, ○) as described in Materials and Methods. EAE scores are shown as mean ± SEM. (C) MOG-specific cytokine production by LN cells from EAE-induced Opn-deficient (□) and Opn WT/HZ (▪) 2D2 TCR transgenic mice. (D) 2D2 CD4+ T cell proliferation from the same set of Opn-WT (•) and Opn-deficient (○) cells in C.