Table 2. Physical and kinetic parameters used in the simulations.
| Parameter | Value | References and notes |
|---|---|---|
| R | 10.00 μm | Ref. 32 |
| [Rec] | 0–50 nM | Ref. 33 |
| [PI3K] | 50.00 nM | Ref. 10 |
| [PTEN] | 50.00 nM | Assumed |
| [PIP2] | 500 nM | Ref. 11; for unstimulated cells |
| D | 0.1–1 μm2/s | Ref. 34 |
| kcatPI3K | 1.00 s–1 | Ref. 10 |
| kcatPTEN | 0.50 s–1 | Ref. 35 |
| KMPI3K | 200.00 nM | The value obtained from ref. 10 was modified to take into account the effect of reduced dimensionality according to Adam and Delbruck (36) |
| KMPTEN | 200.00 nM | Ref. 35 |
| kassRec | 50.00 (s·μM)–1 | Ref. 37 |
| kassPIP2 | 50.00 (s·μM)–1 | Assumed |
| kdissRec | 0.10 s–1 | Ref. 37 |
| kdissPIP2 | 0.10 s–1 | Assumed |