FIG. 2.
Expression of NSP4 by IDC and MDC treated with RV. (A) DC were treated with control or purified RRV (MOI = 5) for 24 h. The cells were then permeabilized and stained with MAbs against NSP4 and CD83. (B) Summary of experiments presented in panel A. Open squares, DC treated with control; closed squares, DC treated with RRV. Results from 11 and 12 experiments with IDC and MDC are presented. The lines represent medians. The asterisk indicates a statistical difference between the frequencies of IDC and MDC expressing NSP4 (P < 0.001; Mann-Whitney test). (C) MDC were infected with RV (MOI = 5) in the presence of the neutralizing anti-VP7 MAb 159 or of an isotype control MAb. (D) MDC were treated with the supernatant of mock-infected or RRV- or Wa RV-infected MA104 cells at an MOI of 5. The results in panels C and D are representative of three experiments performed. The percentages of cells in the in the upper quadrants of the dot plots are indicated.