TABLE 3.
Heparin sulfate column binding of AAV2 virus preparations
| Virus | Nonbound DRPa | Bound DRPb | Percent Bound |
|---|---|---|---|
| AAV2 | 2.4 × 109 | 1.5 × 1010 | 0.86 |
| T70-43 | 2.0 × 1010 | 1.0 × 109 | 0.05 |
| T88-41 | 1.7 × 1010 | 1.4 × 109 | 0.08 |
a
DNase-resistant particle (DRP) copy number was determined using Taqman Q-PCR. Primers and probe were homologous to a conserved cap sequence (see Materials and Methods). Nonbound represent total virus found in wash and flowthrough material. Data shown are the average of two experiments.
b
Copy number present in pooled 1-ml gradient fractions. Data shown are the average of two experiments.