TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Genotype | Reference |
|---|---|---|
| Strains | ||
| E40 | Y. enterocolitica O:9 | |
| MRS40 | blaA luxAB+ mutant of E40 | 60 |
| APB40 | inv::pMS154 mutant of MRS40 | 14 |
| pYV40 plasmids | ||
| pYV40 | WT plasmid of Y. enterocolitica E40 | 60 |
| pAB404 | yopE21yopHΔ1-352 | 13 |
| pAB406 | yopOΔ65-558 | 44 |
| pAB408 | yopM23 | 44 |
| pAB4052 | yopE21 | 44 |
| pAPB4011 | yadAΔ68-326 | 14 |
| pIM409 | yopT135 | 31 |
| pIM424 | yopE21yopT135 | 31 |
| pIM425 | yopHΔ1-352yopT135 | 14 |
| pIM426 | yopE21yopHΔ1-352yopT135 | 14 |
| pIML421 | yopE21yopHΔ1-352yopP23yopM23yopT135yopOΔ65-558 (called ΔHOPEMT) | 31 |
| pMSK41 | yopP23 | 44 |
| pMSL41 | yscNΔ169-177 | 63 |
| pPW401 | yopBΔ89-217 | 13 |
| pSI4008 | yopHΔ1-352 | 44 |
| Expression plasmids | ||
| pIML279 | pBC18R Plac yopT+sycT+ | 31 |
| pMSL34 | pSelect-1 PyopO yopO+ | This work |
| pMSL68 | pBC19R Plac yopE+sycE+ | This work |
| pMSL69 | pBluescriptKS(−) Plac yopH+sycH+ | This work |
| Suicide plasmids | ||
| pINT192 | oriR6K mobRP4 cat; contains 1.4-kb NdeI-BglII internal fragment of inv from Y. enterocolitica 8081 | 48 |
| pMS154 | pKNG160 containing 1.4-kb internal inv fragment subcloned from pINT192 | 64 |